SET OF FLUORESCENTLY LABELLED OLIGONUCLEOTIDE PROBES FOR TYPING STRAINS OF Burkholderia mallei BY METHOD OF AMPLIFICATION OF DIFFERENTIATING DNA FRAGMENTS

摘要

FIELD: biotechnology.;SUBSTANCE: probes included in the set have a structure of a "pin" with fluorophore and fluorescence extinguisher at the ends and have complementarity to products of an amplification reaction with primers. The developed set of hybridisation probes can be used for the fluorescent detection of the results of typing the actinobacillus mallei by a method of amplification of differentiating fragments of a genome in the implementation of the epidemiological surveillance of the glanderous infection.;EFFECT: possibility of simultaneous analysis of nine DFR-loci.;2 dwg, 1 tbl, 3 ex