首页> 外国专利> METHOD OF COLLECTING FERTILISED EGGS (in vitro) FROM EXCITER Fasciola hepatica INTRA VITAM

METHOD OF COLLECTING FERTILISED EGGS (in vitro) FROM EXCITER Fasciola hepatica INTRA VITAM

机译:从体外肝片状筋膜藻中收集受精卵的方法(体外)

摘要

FIELD: biotechnology.SUBSTANCE: method comprises the steps: collecting from the bile ducts of liver of domestic and/or wild animals infected with fasciolas of only live adult F. hepatica. Placement them into individual tubes with filtered and centrifuged bile diluted with isotonic solution of sodium chloride 1:1. Exposure of tubes at t = 38-39°C if F. hepatica is from cattle, and t = 39-40°C if F. hepatica is from sheep and/or goats, under conditions of thermostat for 5 hours. Subsequent washing eggs in isotonic solution of sodium chloride.EFFECT: invention enables to obtain up to 100 percent of fertilised eggs of Fasciola of species Fasciola hepatica and can be used for study in the laboratory or field experiments in solving fundamental and applied scientific tasks in the field of epizootiology, treatment and prevention of fascioliasis of domestic or wild animals.2 ex
机译:领域:生物技术。方法:该方法包括以下步骤:从感染了仅活的成年肝片筋膜的家畜和/或野生动物的肝胆管中收集。将它们放入单独的试管中,过滤并离心的胆汁用氯化钠的等渗溶液1:1稀释。如果将F. hepatica来自牛,则将管暴露于t = 38-39°C,如果将F. hepatica来自绵羊和/或山羊,则将管在t = 39-40°C的条件下恒温5小时。随后在氯化钠的等渗溶液中洗涤鸡蛋。效果:本发明能够获得高达100%的Fasciola hepatica种类的Fasciola受精卵,可用于实验室或野外实验研究,以解决人类中的基础和应用科学任务。家畜或野生动物的动物流行病学,治疗和预防筋膜炎的领域。2 ex

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