METHOD OF TREATMENT OF BLOOD SAMPLE AT IMMUNOLOGICAL ANALYSIS OF ALLERGEN-SPECIFIC AND TOTAL IMMUNOGLOBULINS E

摘要

FIELD: biotechnologies.;SUBSTANCE: before the immunoanalysis the immunoglobulins G are removed from the sample, in particular, by interaction of the sample with the sorbent which is specifically binds immunoglobulins G then the immunological analysis of allergen-specific and total immunoglobulins E is conducted using the allergens or mixes of allergens and antibodies against the immunoglobulins E immobilised in the elements of the biological microchip which is the array of three-dimensional hydrogel elements containing elements with covalently immobilised allergens and antibodies against the immunoglobulin E, while the array elements are microdrops obtained by the polymerisation immobilisation method.;EFFECT: decrease of non-specific signals, increase of the ratio signal-background, increase of reproducibility of the measured specific signals and improvement of sensitivity and reproducibility at analysing for determination of total and allergen - specific immunoglobulins.;3 cl, 2 dwg, 2 tbl, 2 ex