A method for detecting the expression of recombinant proteins on a cell surface and for the immobilization and purification of cells and proteins

摘要

A method for detecting the expression of a target polypeptide produced by means of a recombination in a transformed host cell, wherein the recombinant polypeptide in or on which the host cell against the surrounding outer medium defining cell surface, and one of the outer medium from accessible section, characterized in,a) that the recombinant polypeptide as a fusion protein with a for the expression and representation of the target polypeptide necessary aids - polypeptide constructed in such a way that, in the accessible section has at least once the amino acid cysteine,b) that the host cell during or after the expression of an external medium is stopped, which contains one or more marker substances, which binds specifically to cysteine,c) that the binding reaction between cysteine and the effects or more marker substances,d) that the host cell is then supplied to a process for the detection of the cysteine - marker - compounds, is subjected to,e) and in that the detection of cysteine - marker - compounds as evidence for the presence of the recombinant zielpolypetids and thus for the expression is considered.