A method for irradiating a sample with light and fluorescence light microscope with an illumination unit for irradiating a sample with light

摘要

A method for irradiating a sample with light,– a first input light beam with a beam splitter is divided into two first partial beams,– the two first partial beams, each with a lens in a same focal point in a common focal plane of the lenses, can be focused,– the optical path lengths of the two first partial beams between the beam splitter and the lenses so be adjusted with respect to one another, that the first two partial beams in the common focal point interfere with a first relative phase,– a second input light beam having the same beam splitter is divided into two second partial beams, and– while in the region of the common focal plane, no interference of the first partial beams takes place with the second partial beams,characterized in that the beam splitter a polarization beam splitter (24) is used,– the polarizations of the two first partial beams and the polarizations of the two second partial beams between the polarization beam splitter (24) and the objective (28, 29) to be adjusted to each other in such a way that, in the region of the common focal plane (30) the first partial beams interfere with each other and the second partial beams interfere,– a first polarization of the first input of light beam (13) and a of the first polarization differing polarization of the second input light beam (14), thus, to the polarization beam splitter (24), be matched to that the two second partial beams in the common focal point (22) with a to the first relative phase by π relative phase shifted second interfere.