A method for the quantitative detection of a contamination of a liquid by a microorganism by means of quantitative polymerase chain reaction

摘要

A method for the quantitative detection of a contamination of a liquid comprising an organic compound by a microorganism, comprising: obtaining a solids content of the liquid; isolating a dna from the solids content; amplifying of a specific section of the isolated dna by means of qpcr and obtaining a amplified fragment; determining an absolute mass of the amplified dna - section on the basis of a fluorescence of the amplified fragment; evaluating a degree of microbial contamination of the liquid, optionally comprising a estimating a cell number of the microorganism per unit volume of the liquid; wherein the fluorescence of the sample on a marking of adjacent nucleotides of a dns - extrudate with a fluorescent dye is based, and the amplified specific section of the dna is defined with the aid of a primer - pair comprising a the stated nucleotide sequences: