IN VITRO PRODUCTION OF DNA MINICIRCLES COMPRISING LESS THAN 250 BASE PAIRS

摘要

A method for the in vitro production of DNA minicircles comprising steps of: a) providing nicked double-stranded oligodeoxynucleotides bunt-ended substrates having at least one phosphorylated 5' end, b) performing a ligase-mediated circularization on a reaction mixture comprising the said nicked double-stranded oligodeoxynucleotides substrates and a DNA bending protein, and c) obtaining DNA minicircles.