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In vitro production of DNA minicircles comprising less than 250 base pairs
In vitro production of DNA minicircles comprising less than 250 base pairs
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机译:包含少于250个碱基对的DNA小环的体外生产
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摘要
A method for the in vitro production of DNA minicircles includes steps of: a) providing nicked double-stranded oligodeoxynucleotides bunt-ended substrates having at least one phosphorylated 5′ end, b) performing a ligase-mediated circularization on a reaction mixture including the nicked double-stranded oligodeoxynucleotides substrates and a DNA bending protein, and c) obtaining DNA minicircles.
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