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Intact minicells as vectors for DNA transfer and gene therapy in vitro and in vivo
Intact minicells as vectors for DNA transfer and gene therapy in vitro and in vivo
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机译:完整的小细胞作为体外和体内DNA转移和基因治疗的载体
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摘要
A composition comprising recombinant, intact minicells that contain a therapeutic nucleic acid molecule is disclosed. Methods for purifying a preparation of such minicells also are disclosed. Additionally, a genetic transformation method is disclosed, which comprises (i) making recombinant, intact minicells available that contain a plasmid comprised of a first nucleic acid segment, and (ii) bringing the minicells into contact with mammalian cells that are engulfing-competent, such that the minicells are engulfed by the mammalian cells, which thereafter produce an expression product of the first nucleic acid segment.
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