USE OF ADENOSINE DIPHOSPHATE GLUCOSE PYROPHOSPHORYLASE FOR SEQUENCING A NUCLEIC ACID MOLECULE

摘要

The invention provides for sequencing a nucleic acid molecule based on the detection of base incorporation by the release of pyrophosphate (PPi) using a new enzyme system comprising adenosine diphosphate (ADP)-glucose pyrophosphorylase (AGPase) and its substrate ADP-glucose.