METHODS OF DETECTING NUCLEIC ACIDS IN INDIVIDUAL CELLS AND OF IDENTIFYING RARE CELLS FROM LARGE HETEROGENEOUS CELL POPULATIONS

摘要

The invention provides a method of detecting a nucleic acid target within a cell in a sample, the method comprising labeling the nucleic acid using at least two capture probes that capture a label probe to the nucleic acid target. The signal may be amplified with a help of one or more amplifiers and preamplifiers. The method is useful inter alia for identifying a cell of a certain type in a mixture of cells based. The invention further provides a kit for carrying out the method of the invention as well as a composition and a tissue section comprising a fixed and permeabilized cell with the labeling system as identified for the method of the invention.