Method of lyophilizing encapsulated cells, lyophilized encapsulated cells, compositions comprising lyophilized encapsulated cells, and uses of such cells and compositions

摘要

The present disclosure is a method of lyophilizing encapsulated cells, comprising at least two successive incubation steps, wherein the encapsulated cells are incubated for an appropriate amount of time in an incubation solution containing a cryoprotectant in each incubation step. Providing a method wherein the concentration of cryoprotectant in the incubation solution is increased in each subsequent incubation step. The present disclosure also provides lyophilized cells obtained by this method and various uses of these cells for use as pharmaceuticals, food additives or cosmetic additives To do. The present disclosure also provides a composition comprising skim milk, glycerol and a carbohydrate.