Method and apparatus for the synthesis of cell-free proteins using eukaryotic cell lysates in the presence of caspase inhibitors, and the use of caspase inhibitors to increase the yield and / or stability of proteins synthesized in the method

摘要

The present invention uses an improved method for the synthesis of cell-free proteins using a eukaryotic cell lysate in the presence of a caspase inhibitor, an apparatus for performing this method, and a eukaryotic cell lysate. In a cell-free method for the synthesis of proteins, it relates to the use of caspase inhibitors for increasing the yield of proteins and / or for increasing the stability of the synthesized proteins. In a preferred embodiment of the invention, the method is carried out in an apparatus comprising at least two compartments separated by a dialysis membrane, the translation reaction is carried out in at least one first compartment, the reaction compartment, During which i) the reactant diffuses through the dialysis membrane into at least one additional compartment, the supply / exhaust compartment, into the reaction compartment, and ii) the reaction by-product diffuses through the dialysis membrane from the reaction compartment into the supply / exhaust compartment. To do. According to the present invention, the caspase inhibitor may preferably be present in the supply / exhaust compartment in order to further supply fresh inhibitor to the reaction compartment, but it is present at least in the reaction compartment. [Selection figure] None