A method and apparatus for cell-free protein synthesis using a eukaryotic cell lysate in the presence of a caspase inhibitor, and use of a caspase inhibitor to increase the yield and / or the stability of the synthesized proteins in such a method

摘要

The invention relates to an improved method for cell-free protein synthesis using a eukaryotic cell lysate in the presence of a caspase inhibitor, an apparatus for carrying out this method and the use of a caspase inhibitor to increase the protein yield or to increase the stability of the synthesized proteins in such a cell-free method for protein synthesis using a eukaryotic cell lysate. In a preferred embodiment of the invention, the method of cell-free protein synthesis is carried out as a continuous process in a device comprising at least two compartments separated by a dialysis membrane, wherein the translation reaction takes place in at least a first compartment, the reaction compartment, and during the translation reaction through the dialysis membrane i) Reactants from at least one further compartment, the supply and disposal compartment, diffuse into the reaction compartment and ii) diffuse reaction by-products from the reaction compartment into the supply and disposal compartment. According to the invention, the caspase inhibitor is present at least in the reaction compartment, but may preferably also be present in the supply and disposal compartment in order to supply unused inhibitor to the reaction compartment.