Method of detecting microRNA target gene detection kit and microRNA target gene

摘要

providing a detection kit for detecting a target gene of miRNA. Furthermore, without the need to perform the operation of introducing the gene via a vector into a cell, there is provided a method of detecting a target gene of miRNA easily. Includes labeling microRNA or microRNA for labeling reagent and cell extraction reagent, by the target gene detection kit for microRNA comprising the reaction reagents and microRNA labeling substance further. By to prepare a cell extract with mild conditions, was added miRNA that was labeled cell extract to recover the labeling substance can be recovered mRNA corresponding to the target gene of miRNA easily. To prepare a cDNA from the mRNA was recovered, and detects the target gene miRNA.