A method for identifying a cell in which the intracellular concentration of a specific metabolite is increased compared to its wild type, wherein the modification of the cell is achieved by recombinant manipulation, as well as optimally producing the specific metabolite, Method for producing production cells genetically modified compared to wild type, method for producing this metabolite, and nucleic acid suitable therefor

摘要

The present invention relates to a method for identifying a cell in which the intracellular concentration of a specific metabolite is increased compared to its wild type, wherein the modification of the cell is achieved by recombinant manipulation, as well as to optimize the specific metabolite. The present invention relates to a method for producing a production cell that is genetically modified compared to its wild type, a method for producing this metabolite, and a nucleic acid suitable therefor. According to the present invention, a gene encoding a recombinase that is homologous to a known recombinase gene is transformed into cells via a vector, and these cells are transformed into at least one modified gene G1-Gn or at least one DNA containing the mutations M1-Mn is inserted into the cell, and that cell that exhibits the highest metabolite production is identified using a metabolite sensor. From these cells, the mutation responsible for increased production is isolated, the gene or mutation is removed and inserted into the production strain, whereby the production strain exhibits increased production of metabolites.