Use of solubilized Klotho, FGF23 and FGFR complex formation mechanism in calcified tissue

摘要

Provided are a method for screening an agent for controlling calcification, to which signal transduction relates, common to hard tissue and/or blood vessel; a calcification controlling agent; a method for examining calcification of a bone, calcification of a blood vessel and the pathological advance of a disease accompanying the same; and an examination kit therefor. The method for screening an agent for controlling calcification of hard tissue and/or blood vessel according to the first aspect comprises a step for, in a specimen in the absence and presence of a test compound, contacting soluble Klotho with FGFR and FGF23, and a step for selecting a compound causing a change in the specific signal transduction. The calcification controlling agent according to the second aspect comprises a compound capable of inhibiting or promoting the formation of a soluble Klotho-containing complex. The method for examining calcification according to the third aspect comprises a step for measuring the concentration of soluble Klotho, etc. The kit for examining calcification according to the fourth aspect contains a ligand having binding affinity to soluble Klotho, etc.