A method for rapidly inducing mesenchymal stem cells to convert to hematopoietic stem cells on a large scale with high purity

摘要

The present invention presents a method which can rapidly induce large-scale and high-purity mesenchymal stem cells to transdetermine into hematopoietic stem cells, includes the steps as follows: 1)preparation of homogeneous medium of mesenchymal stem cells; 2)Combination of a plurality of small RNA molecules; 3)assembling and transfection of nanoparticles of nucleic acids and polypeptides; 4)inducing and amplifying medium of post-transdetermined hematopoietic stem cells; 5)activating multiple hemopoiesis-related genes. It resolves the technical bottleneck problem of cell matching difficulties, immune rejection and quantity limitation of hematopoietic stem cells in prior art in hematopoietic stem cell treatment.