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Method for Detection of Pseudomonas Aeruginosa (PA) Using Volatile Biomarkers

机译:挥发性生物标记物检测铜绿假单胞菌的方法

摘要

A method of determining the presence of Pseudomonas involves establishing a gaseous headspace over a surface suspected of containing at least one Pseudomonas strain and contacting at least a portion of the gaseous headspace with a capillary microextraction of volatiles (CMV) sampling device to absorb at least one component of the headspace by the CMV sampling device. The component loaded CMV sampling device is coupled to an injection port of an analytical device where the components are desorbed into the analytical device, where components are separated, detected, and identified to determine if one or more of the identified components is a biomarker for at least one Pseudomonas strain.
机译:一种确定假单胞菌存在的方法,涉及在怀疑包含至少一种 Pseudomonas 菌株的表面上建立气态顶空,并使至少一部分气态顶空与A接触。毛细管微萃取挥发物(CMV)采样装置,以通过CMV采样装置吸收顶空的至少一种成分。加载有组分的CMV采样装置连接至分析装置的注入口,在该处将组分解吸到分析装置中,在其中分离,检测和鉴定组分,以确定一种或多种被鉴定的组分是否为至少一种生物标记。至少一种假单胞菌菌株。

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