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COMPOSITIONS AND METHODS CONTAINING A CONSTITUTIVE PROMOTER TO MODIFY THE EXPRESSION OF GENES OF INTEREST IN PLANTS

机译:包含组成型启动子以修饰植物中兴趣基因表达的组合物和方法

摘要

The present invention relates to a new promoter of the expression of genes in plants. More specifically, the invention relates to regulatory sequences of polynucleotides that are isolated from soy plants and can initiate and activate polynucleotide transcription, and to the use of these regulatory sequences to modify the transcription of endogenous and/or heterologous polynucleotides and to produce polypeptides. The invention further describes DNA constructs that contain the promoter of the gene of the supposed flavonol-sulfotransferase protein in soy plants, which promoter is functionally linked to a heterologous and/or endogenous gene. Moreover, the invention relates to the use of these constructs in the form of expression cassettes, expression vectors, recombinant vectors and in plants, plant cells or transgenic protoplasts. The invention also describes a method using such constructs that contain the promoter of the gene of the putative flavonol-sulfotransferase protein of soy plants for the production of transgenic plants, plant cells or protoplasts.
机译:本发明涉及植物中基因表达的新启动子。更具体地说,本发明涉及从大豆植物中分离并能启动和激活多核苷酸转录的多核苷酸的调控序列,并涉及这些调控序列在修饰内源和/或异源多核苷酸的转录并产生多肽中的用途。本发明进一步描述了DNA构建物,其包含大豆植物中假定的黄酮醇-磺基转移酶蛋白的基因的启动子,该启动子功能性地连接于异源和/或内源基因。此外,本发明涉及这些构建体以表达盒,表达载体,重组载体的形式以及在植物,植物细胞或转基因原生质体中的用途。本发明还描述了一种使用这样的构建体的方法,该构建体包含大豆植物推定的黄酮醇-磺基转移酶蛋白的基因的启动子,用于生产转基因植物,植物细胞或原生质体。

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