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Compositions and methods containing a constitutive promoter to modify the expression of genes of interest in plants

机译:含有组成促进剂的组合物和方法,以改变植物中感兴趣的基因的表达

摘要

The present invention relates to a new promoter of the expression of genes in plants. More specifically, the invention relates to regulatory sequences of polynucleotides that are isolated from soy plants and can initiate and activate polynucleotide transcription, and to the use of these regulatory sequences to modify the transcription of endogenous and/or heterologous polynucleotides and to produce polypeptides. The invention further describes DNA constructs that contain the promoter of the gene of the supposed flavonol-sulfotransferase protein in soy plants, which promoter is functionally linked to a heterologous and/or endogenous gene. Moreover, the invention relates to the use of these constructs in the form of expression cassettes, expression vectors, recombinant vectors and in plants, plant cells or transgenic protoplasts. The invention also describes a method using such constructs that contain the promoter of the gene of the putative flavonol-sulfotransferase protein of soy plants for the production of transgenic plants, plant cells or protoplasts.
机译:本发明涉及植物中基因表达的新推动者。更具体地,本发明涉及从大豆植物中分离的多核苷酸的调节序列,并且可以引发和激活多核苷酸转录,并使用这些调节序列来改变内源和/或异源多核苷酸的转录并产生多肽。本发明还描述了含有大豆植物中所假发的黄酮酚 - 磺基转移酶蛋白质的基因的启动子的DNA构建体,该启动子在功能上与异源和/或内源基因连接。此外,本发明涉及这些构建体以表达烧结,表达载体,重组载体和植物,植物细胞或转基因原生质体的形式使用。本发明还描述了一种方法,该方法使用该构建体含有含有大豆植物的推定的黄酮 - 磺旋转酶蛋白的基因的启动子,用于生产转基因植物,植物细胞或原生质体。

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