SCREENING METHOD FOR ION CHANNEL MODULATORS USING MUTATED BKCA CHANNEL

摘要

The present disclosure relates to a doubly mutated BK_Ca channel construct and a novel cell-based screening system for ion channel modulators using the same. A doubly mutated BK_Ca channel-comprising cell-based system of the present invention, when compared to a control group, shows remarkably increased fluorescence caused by membrane depolarization, regardless of whether there is a separate increase of [Ca²⁺]_i, and shows activity (movement in the negative direction of a G/V curve) that is further triggered by a known activator (for example, CTBIC). Therefore, the system of the present disclosure is capable of more effectively and accurately analyzing the activity of an ion channel, and thus can be effectively applied not only to the separation/identification of ion channel modulators but also to screening for a therapeutic agent for a condition, disease or disorder related to the modulation of a BK_Ca channel.