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Ferulate esterase isolated from Lactobaccillus fermentum
Ferulate esterase isolated from Lactobaccillus fermentum
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机译:从发酵乳杆菌中分离出阿魏酸酯酶
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摘要
The cDNA and amino acid sequences of a ferulate esterase obtained from Lactobacillus fermentum NRRL B-1932 is determined. An expression vector for expression of the ferulate esterase gene is generated. The recombinate ferulate esterase gene and transcribed protein contains a linker sequence and 6×HIS tag for purification. Enzymatic activity of the recombinant protein is determined.
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