An isolated nucleic acid molecule comprising a polymorphic site selected from the group consisting of positions 164, 269, 284, 407 and 989 of SEQ ID NO: 1, an array or a kit comprising the same. Also provided are a method for detecting single nucleotide polymorphism (SNP) in bovine proteinase inhibitor (PI) gene, a method for haplotyping a bovine cell, a method for progeny testing of cattle based on said haplotyping, a method for selectively breeding of cattle based on haplotyping a parent animal. The present invention further provides a method for testing a dairy cattle for its milk production trait, comprising haplotyping its cells, wherein a cattle having haplotypes 1, 3, 4 or 5 indicates that the cattle has desirable milk production trait. Haplotype 1 indicates that the cattle has the most desirable milk production trait.
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机译:分离的核酸分子,其包含选自SEQ ID NO:1的位置164、269、284、407和989的多态性位点,包含其的阵列或试剂盒。还提供了用于检测牛蛋白酶抑制剂(PI)基因中的单核苷酸多态性(SNP)的方法,用于牛单倍型的方法,基于所述单倍型的牛后代测试的方法,基于牛的选择性繁殖的方法单倍体亲本动物。本发明进一步提供了一种测试奶牛的产奶性状的方法,包括对其细胞进行单倍型分析,其中具有1、3、4或5单倍型的牛表明该牛具有理想的产奶性状。单倍型1表明牛具有最理想的产奶特性。
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