African swine fever virus Defective in CD2 as subsequently attenuated Live vaccine or Inactivated vaccine against African Swine Fever in Mammals

摘要

An African swine fever virus (asfv) preferably Live Attenuated or Inactivated subsequently, which comprises a CD2 genomic Gene non functional, such asfv is not deficient in its Replication, as well as immunogenic compositions and Vaccines correspondient or compositions It is,Production methods and uses for the treatment and Prevention of African Swine Fever in Mammals of the Family Suidae, preferably, for example, Pigs, more preferably domestic Pigs (Sus scrofa domu00e9sticus), Wild Boar (Sus scrofa scrofa), Wild Boar (Warty Potamochoerus porcus), Rio (River Hogs, Potamochoerus larvatus)Giant Forest Hogs (hilochoerus meinertzhageni) and wild boars. Claim 2: The asfv according to claim 1, wherein the Gene is not ep402r CD2 genomic functional and / or includes, and preferably consists of a Nucleic Acid Sequence according to the SEQ ID no. 1.Claim 8: a method for the generation of a non Functional asfv CD2 Gene in a Genome asfv,Characterized in that it comprises the steps of: (a) introducing one or more complete or partial Gene deletions in the CD2 asfv and / or modify one or more nucleotides that Control and / or the product of gene encoding CD2 asfv and / or disrupt the open reading frame (ORF) of CDE Asfv thus CD2 asfv is not FunctionalPreferably by the Lac Repressor i together with the Marker Gene of B - glucuronidase in the Locus of CD2 asfv, leading to a nearly complete deletion of the Gene asfv CD2, making this mode is not functional in vitro or in vivo.