obtaining bioactive secondary compounds of phoma herbarium endophyte with broad spectrum antimicrobial activity

摘要

obtaining bioactive secondary compounds of phoma herbarium endophyte with broad spectrum antimicrobial activity. The endophytic fungus phoma herbarium, isolated from the medicinal plant luehea divaricata, is a non-pathogenic fungus from humans, producing effective secondary metabolites compared to a commercial fungicide against the alternaria alternata phytopathogenic fungi, guignardia citricarpa and fusarium solani f. sp. glycines. It has action against the bacteria escherichia coli, micrococcus luteus, salmonella typhi, human pathogens and phytopathogenic bacteria xantomonas asc. phaseoli. The invention assists in the control of plant pathogenic fungi such as cocoa and soybean as well as assisting in the control of human and plant pathogenic bacteria. Moreover, this invention is an alternative to the use of agrochemicals, as it is a natural product, easy to handle, easy to produce in the environmentally friendly environment. The amount of secondary metabolites obtained from the total fermentation of the potato-dextrose medium by the pophy herbarium endophytic fungus is usually around 120mg per liter of fermented medium. From 300 mg of concentrated fungal crude extract (metabolites), 2.6 to 47.1 mg of bioactive metabolites with antimicrobial action are obtained, and in laboratory tests 11 to 188.5 are required. µg / ml metabolites for effective control of plant pathogenic fungi.