The methodology of assessment of the toxicity of oncofu00e1rmacos and nanostructures in oocytes and embryos of cattle

摘要

Summary of the invention patent: "methodology for assessment of toxicity of oncofu00e1rmacos and nanostructures in oocytes and embryos of cattle", the present invention relates to the methodology of analysis of toxicity caused by new oncofu00e1rmacos and nanostructures based on assessments Oocytes and embryos, bovine, and more particularly,Refers to the methodology used as diagnosis model of the harmful effect caused by drugs in tumour cells does not use human cancer, serving as a support for the in vitro tests of effectiveness of the active principle.The present invention has as main objective to contribute to technological development in the therapy of cancer, from the identification of new drugs with potential antitumor. The present invention includes that the methodology of analysis of cellular toxicity caused by these oncofu00e1rmacos and nanostructures, based on assessments of in germ cells and embryonic stem cells.In particular, we propose a sequence of assessment methods, allowing a selection of formulations and concentrations of the oncofu00e1rmacos and nanostructures still in the stage of in vitro evaluation.A battery of tests of in vitro development; followed by staining to determine the viability and integrity of cell membrane, formation of reactive oxygen species, stage of the cell cycle and apoptosis, analysis of gene expression related to the processes of apoptosis, EST Reese oxidative and cell growth; thus,The diagnosis of deleterious effects caused by tumor cells does not effectively, quickly and efficiently in the laboratory of cellular culture and embryology, increasing the in vitro tests pre - clinical oncofu00e1rmacos and nanostructures. 1 / 2