The non competitive immunoassay for small analytes

摘要

"The non competitive immunoassay for small analytes. The invention relates to a non competitive immunoassay for small analytes, where the analyte is reacted with two partners ligands.The first partner ligand binds to the analyte to form a complex between the first partner ligand and the analyte, and the second partner ligand binds to the complex formed by the first partner ligand and the analyte. The resulting complex formed between the analyte and binding partners is detected. The partners ligands are proteins, such as antibodies, including antibody fragments.The invention also relates to reactive pairs and test kits useful in tests, as well as the use of reactive pairs and a process for their preparation, new reagents and means for their preparation are also provided.