Hyaluronidase protein identified as present in the venom of Loxosceles intermedia, cloned and expressed recombinant form in heterologous expression system

摘要

Hyaluronidase protein identified as present in the venom of Loxosceles intermedia.Cloned and expressed recombinant form in heterologous expression system, the present invention patent covers the uses and applications of recombinant toxin from the venom Loxosceles infermedia characterized as hioluronidase - class of enzymes has been described as important regul You love to several critical aspects of cell physiology and biochemistry.Both physiological processes and pathological. The hialuronidases are a group of enzymes that mediate the degradation of acid hialuru00e2nico, and thus these enzymes are able to increase the permeability of connective tissues and decrease the viscosity of the fluids.The present invention includes the following uses and applications of the toxin recombinant hyaluronidase Loxosceles intermedia (lipechyase) obtained by means of the expression system heteru00f3logos: (a) as reagent hey or molecular models for biological and biochemical studies in vitro and in vivo, And their biotechnological applications in these areas: (b) as a reagent in biochemical and biological studies of di...An agnostic and own clinical diagnostic laboratory of pathology and loxoscelismo biotechnology and its application in this area; (c) for the detection of enzymes related to and as models for development of detection methods of these.