首页>
外国专利>
PROCESS FOR COUNTING OF MAMMALS CELL micronuclei WITH A HEAD WEIGHT OF DIFFERENTIAL STAINING OF micronuclei AND chromatin OF DEAD AND DYING CELLS
PROCESS FOR COUNTING OF MAMMALS CELL micronuclei WITH A HEAD WEIGHT OF DIFFERENTIAL STAINING OF micronuclei AND chromatin OF DEAD AND DYING CELLS
展开▼
机译:头重于死细胞和死细胞的微核和染色质差异染色的哺乳动物细胞微核的计数过程
展开▼
页面导航
摘要
著录项
相似文献
摘要
The present invention relates a method for the enumeration of mammalian cell micronuclei, while distinguishing micronuclei from the chromatin of dead and dying cells. The method utilizes differential staining of chromatin from dead and dying cells, to distinguish the chromatin from micronuclei and nuclei that can be detected based upon fluorescent emission and light scatter following exposure to an excitatory light source. Counting of micronuclei events relative to the number of nuclei can be used to assess the DNA-damaging potential of a chemical agent, the DNA-damaging potential of a physical agent, the effects of an agent which can modify endogenously-induced DNA damage, and the effects of an agent which can modify exogenously-induced DNA damage. Kits for practicing the invention are also disclosed.
展开▼