CONSTRUCTION AND APPLICATION OF MUTANT TYPE B HUMAN ADENOVIRUS AD11 HAVING ENHANCED ONCOLYTIC POWER

摘要

A method for constructing type B recombinant human adenoviral vectors Ad11 5EP and Ad11 5ETel GFP and applications thereof. Construction of the adenoviral vector Ad11 5EP utilizes homologous recombination to substitute a 365 bp long gene upstream segment of the Ad5 E1A coding sequence (comprising the enhancer and promoter of Ad5 E1A) to the corresponding region of type B human adenovirus subtype 11 Ad11 thereby constructing said vector Ad11 5EP. The method for constructing the type B recombinant human adenoviral vector Ad11 5ETel GFP is simultaneous homologous recombination of the genomes of the constructed shuttle vectors pSSENTel pSSGFP and Ad11 5EP so as to produce said Ad11 5ETel GFP. Vector Ad11 5EP possesses a stronger oncolytic effect than the wild type Ad11 which enhances the power to kill tumor cells. Measurements of tumour growth and tumour clearance rates show that Ad11 5EP effectively reduces tumour growth rates while the tumour free ratio among tumour bearing mice using Adll 5EP is noticeably better than for Ad11. Constructed Ad11 5ETel GFP can be used for treating tumours or for specific sensitive and economical detection of tumour cells in circulating blood.