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ISOLATING AND IDENTIFYING THE ACTIVE CONSTITUENT OF ETHANOLIC EXTRACT OF CAYRATIA IRIFOLIA, BEING A CONSTITUENT FOR THE TREATMENT OF OVARIAN CANCER
ISOLATING AND IDENTIFYING THE ACTIVE CONSTITUENT OF ETHANOLIC EXTRACT OF CAYRATIA IRIFOLIA, BEING A CONSTITUENT FOR THE TREATMENT OF OVARIAN CANCER
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机译:分离和鉴定伊卡利亚夜蛾的乙醇提取物的活性成分,是治疗卵巢癌的成分
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摘要
A process for isolating and identifying the active constituent of ethanolic extract of Cayratia trifolia, being a constituent for the treatment of ovarian cancer, comprising the steps, in combination, of subjecting samples of the said extract to Column chromatography and Thin layer column chromatography for the separation of active constituents; Fourier transforms infrared (FTIR) spectroscopy for analysis of the phytoconstituents functional groups for identifying the functional groups; identification of the structure of the active constituents by Nuclear Magnetic Resonance (NMR) spectroscopy: two dimensional nuclear magnetic resonance (2D NMR) spectroscopy for resolving and identifying the nature of chemical sites in the sample; HSQC (Heteronuclear Single Quantum Coherence) for the detection of bond couplings; H - H COSY (Homonuclear Correlation Spectroscopy) for revealing correlations between coupled protons; HMBC (Heteronuclear Multiple bond Coherence) also for the detection of bond couplings; the overall analysis indicating a structure of the active constituent of the said extract with a molecular formula = C13H32O, molecular weight equal to 264.44 grams / mole, compound name: Linolenyl Alcohol.
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机译:一种用于分离和鉴定三叶草的乙醇提取物的活性成分的方法,该成分为治疗卵巢癌的成分,包括以下步骤:组合地,对所述提取物的样品进行柱色谱法和薄层柱色谱法处理。分离有效成分;傅里叶变换红外(FTIR)光谱用于分析植物成分官能团,以鉴定官能团;通过核磁共振(NMR)光谱鉴定活性成分的结构:二维核磁共振(2D NMR)光谱,用于解析和鉴定样品中化学位点的性质; HSQC(异核单量子相干),用于检测键耦合; H-H COZY(同核相关光谱法),用于揭示耦合质子之间的相关性; HMBC(异核多键相干性)还用于检测键耦合;总体分析表明,所述提取物的活性成分的结构具有分子式= C 13 H 32 O,分子量等于264.44克/摩尔,化合物名称:亚麻醇。
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