ISOLATING AND IDENTIFYING THE ACTIVE CONSTITUENT OF ETHANOLIC EXTRACT OF AERVA LANATA, BEING A CONSTITUENT FOR THE TREATMENT OF CARDIAC NECROSIS

摘要

A process for isolating and identifying the active constituent of ethanolic extract f Aerva lanata, being a constituent for the treatment of cardiac necrosis, comprising the steps, in combination, of subjecting samples of the said extract to High Performance Thin Layer Chromatography (HPTLC) for indicating the flavonoid, polyphenol, glycoside, tannin and terpenoid profiles; Column chromatography and Thin layer column chromatography for the separation of active constituents; Fourier transforms infrared (FTIR) spectroscopy for analysis of the phytoconstituents5 functional groups for identifying the functional groups; identification of the structure of the active constituents by Nuclear Magnetic Resonance (NMR) spectroscopy: two dimensional nuclear magnetic resonance (2D NMR) spectroscopy for resolving and identifying the nature of chemical sites in the sample; HSQC (Heteronuclear Single Quantum Coherence) for the detection of bond couplings; *H - *H COSY (Homonuclear Correlation Spectroscopy) for revealing correlations between coupled protons; HMBC (Heteronuclear Multiple bond Coherence) also for the detection of bond couplings; the overall analysis indicating a structure of the active constituent of the said extract with a molecular formula = C12H24O2, molecular weight equal to 200.18 grams/mole, compound name: dodecanoic acid.