ISOLATING AND IDENTIFYING THE ACTIVE CONSTITUENT OF ETHYL ACETATE LEAF EXTRACT OF ALPINIA PURPURATA, BEING A CONSTITUENT FOR THE TREATMENT OF OVARIAN CANCER

摘要

ABSTRACT OF THE INVENTION The process for isolating and identifying the active constituent of ethyl acetate leaf extract of Alpinia purpurata, being a constituent for the treatment of Ovarian cancer, according to this invention, comprises the steps, Column chromatography and Thin layer column chromatography for the separation of active constituents; Fourier transforms infrared (FTIR) spectroscopy for analysis of the phytoconstituents functional groups; identification of the structure of the active constituents by Nuclear Magnetic Resonance (NMR) spectroscopy: two dimensional nuclear magnetic resonance (2D NMR) spectroscopy for resolving and identifying the nature of chemical sites in the sample; HSQC (Heteronuclear Single Quantum Coherence) for the detection of bond couplings; H - H COSY (Homonuclear Correlation Spectroscopy) for revealing correlations between coupled protons; HMBC (Heteronuclear Multiple Bond Coherence) also for the detection of bond couplings; the overall analysis indicating a structure of the active constituent of the said extract with a molecular formula = CtOs, Molecular weight: 312.49 grams/mole and the Compound name: (4-hydroxycyclohexyl) methyl dodecanoate.