A transformed plant comprising stably incorporated in its genome recombinant, or randomly mutagenized rice ahasl1 and a method of producing the same

摘要

The present invention relates to a transformed plant comprising stably incorporated in its genome recombinant, or randomly mutagenized rice AHASL1 a polynucleotide construct comprising a nucleotide sequence operably linked to a promoter that drives expression in a plant cell, wherein said nucleotide sequence is selected from the group consisting of: (a) the nucleotide sequence set forth in SEQ ID NO: 1 or 3; (b) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO: 2; (c) nucleotide sequences encoding a variant rice AHASL1 protein comprising an aspartate or valine substitution at amino acid position 179 or an equivalent position relative to the amino acid sequence set forth in SEQ ID NO: 2 said substitution having resulted from random chemical mutagenesis; (d) the nucleotide sequences according to (a)–(c), further comprising at least one amino acid substitution selected from the group consisting of: (i) the alanine at amino acid position 96 or an equivalent position relative to the amino acid sequence set forth in SEQ ID NO:2 is substituted with threonine, (ii) the proline at amino acid position 171 or an equivalent position relative to the amino acid sequence set forth in SEQ ID NO:2 is substituted with alanine, threonine, histidine, leucine, arginine, isoleucine, glutamine, or serine, (iii) the tryptophan at amino acid position 548 or an equivalent position relative to the amino acid sequence set forth in SEQ ID NO:2 is substituted with leucine, and (iv) the serine at 627 or an equivalent position relative to the amino acid sequence set forth in SEQ ID NO:2 is substituted with asparagine, threonine, or phenylalanine; and (e) nucleotide sequences that are fully complementary to any one of the nucleotide sequences set forth in (a)–(d); wherein said transformed plant has increased tolerance to at least one herbicide relative to the tolerance of an untransformed variety of said plant to said herbicide.