Process for enhancing the amylase inhibitory efficacy from phaseolus vulgaris extracts

摘要

An improved method of purifying bean extract alpha-amylase material is disclosed. The materials purified by this process provide significantly more amylase inhibition than materials purified by other processes. In this process: (a) bean extract alpha-amylase material is suspended in an aqueous solution at a solution pH of from about 3.0 to about 3.5; (b) a preservative is added to the solution; (c) the solution is heated to about 175F to about 195F for from about 10 to about 120 seconds; (d) the solution is cooled to a temperature of from about 65 to about 85F, and the solution is allowed to sit, under static non-agitated conditions, at a temperature with that defined range for from about 3 to about 24 hours to allow bean extract particles to settle out of solution; and (e) the particles are separated from the supernatant.