METHOD FOR OBTAINING A SPRAY ON CELLULAR COMPOUND OF HUMAN FIBROBLASTS AND KERATINOCYTES IN SOLUTION AND THE USE THEREOF AS A REGENERATIVE AGENT IN SKIN LESIONS

摘要

stndrdThe invention relates to a process for obtaining a spray on cellular compound of human fibroblasts and keratinocytes and to the use thereof as a regenerative agent in skin lesions mostly diabetic ulcers and 1 2 and 3 degree burns and as a substitute for flap surgery. The method for obtaining a spray on cellular compound of human fibroblasts and keratinocytes comprises the steps of: isolating the dermis and epidermis incubating the epidermal layer disintegrating the dermal layer and proliferating the fibroblasts disintegrating the epidermal layer proliferating the keratinocytes in suspension preparing the solution of crosslinking agent solution for reacting with the spray on cellular solution and preparing the spray on cellular solution. Said spray on cellular compound of human fibroblasts and keratinocytes increases the effectiveness and ease of application and is intended to be an economical rapid and easy to use alternative.