Method for the quantitative analysis of samples, preferably those of biological origin

摘要

method for quantitative analysis of samples, particularly biological and / or environmental, that are added to the sample solution as internal standard compound, and that the resulting solution is placed at the runway of a porous material and is pr zepu0142yw eluent solution by sample and porous material, characterised by the fact that apply the eluate.which provides the same way migration in porous material for forming internal connection standard and for substances or mixtures of substances as measured, preferably when the relationship determined and internal standard and front show identical paths of migration of the eluent.so that the internal standard substance or mixture of substances to be determined and formed a single zone outside the place of runway, of which a single area or part thereof is entirely standard and internal substance / e / e determined to devices for ana liza instrumental, favourably chromatograph cieczowego and / or mass spectrometer (10),enabling direct determination of substances in the sample in a known manner and the internal standard method, the qualitative and quantitative composition of the eluent is designated by measurements of substances as / s and internal standard in the system of porous material and components of the eluent of different composition quantitative and qualitativein which the substance / e determined / e and standard internal show delay coefficient, used in chromatography, rf = 1,0 or slightly less than 1.0.