PROCESS FOR OBTAINING ANTI-2,4-DICHLOROPHENOXYACETIC ACID (2,4D) ANTIBODIES FROM COMPLEX NANOIMMUNOSORBENT-BASED PROTEIN MIXTURES

摘要

The invention relates to a process for obtaining anti-2,4-dichlorophenoxyacetic acid (2,4D) antibodies meant to be used in immunochemical techniques for dosing 2,4D from environment samples. According to the invention, the process consists in the following stages: synthesis of the nanoimmunosorbent made of SiOnanoparticles whereto the conjugate goat serum albumin (2,4) is coupled, separation of γ globulins from polyclonal antisera containing anti-2,4D specific antibodies and anti bovine serum albumin antibody, the reaction of the anti-2,4D specific antibody from the γ globulin mixture with the 2,4D antigen coupled to the nanoimmunosorbent surface, centrifugation of suspension, dissociation of 2,4D-antigen - anti-2,4D-antibodies immune complex, dosing the anti-2,4D-antibodies amount and storage thereof for use.