Evaluation of 2,4-Dichlorophenoxyacetic Acid (2,4-D), 2,4,5-Trichlorophenoxyacetic Acid (2,4,5-T), and 2,3,7,8-Tetrachlorodibenzo-P-Dioxin (TCDD) Toxicity in C57BL/6 Mice: Reproduction and Fertility in Treated Male Mice and Evaluation of Congenital Malformations in Their Offspring

摘要

The study was undertaken to determine the effects of mixtures (simulated Agent Orange) of 2,4-dichlorophenoxyacetic acid (2,4-D), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on reproduction and fertility of treated male mice. Male C57BL/6 mice were given feed containing varying concentrations of 2,4-D, 2,4,5-T and TCDD such that daily doses of approximately 40 mg/kg 2,4-D, 40 mg/kg 2,4,5-T and 2.4 micrograms/kg TCDD (Group II) or 40 mg/kg 2,4-D, 40 mg/kg 2,4,5-T and 0.16 micrograms/kg TCDD (Group IV) or 20 mg/kg 2,4-D, 20 mg/kg 2,4,5-T aand 1.2 micrograms/kg TCDD (Group III) would be achieved. Controls (Group I) were given a diet with only the corn oil vehicle added to the feed. In the treated animals, dose-related liver and thymus toxicity were found and body weight gain was significantly reduced.