Electrochemical immunosensor for the quantitative determination of mycotoxin fb1

摘要

Electrochemical for quantitative determination of mycotoxin FB1 immunosensor. # The present invention relates to a kit comprising an electrochemical immunosensor for the quantitative determination of fumonisin B1. This is a competitive electrochemical immunosensor direct where specific antibodies FB1 mycotoxin immobilized on magnetic microparticles functionalized with Protein G fumonisin B1 from the test sample will compete with a fumonisin B1 labeled with horseradish peroxidase are used (conjugated FB1 -HRP) for biorecognition element, which is the monoclonal antibody immobilized on the magnetic particles. The step of the enzymatic reaction and electrochemical detection occurring on a multiple electrode, preferably 8 screenprinted carbon electrodes, electrochemically detecting electrons generated after enzymatic reaction. Furthermore, the invention relates to the electrochemical detection method and quantification mycotoxin fumonisin B1 (FB1) using the kit and their use for the detection and quantification in cereals and cereal products.