Method of surface chemical activation of a solid support based on silicon by direct covalent attachment of at least one biomolecule nucleic acid.

摘要

A method of surface chemical activation of a solid support in silicon base by direct covalent attachment of at least one biomolecule nucleic acid. # The present invention relates to a method of surface chemical activation by thiol-ene reaction (TEC) or thiol-yne (WC) of a solid support in silicon base by direct covalent attachment of at least one biomolecule is an oligonucleotide sequence of DNA or RNA, wherein a) the substrate surface is functionalized alkene or alkyne groups and the biomolecule has a group terminal thiol, and / or b) the support surface is functionalised with thiol groups and the biomolecule has a terminal alkene or alkyne group; said method comprising the steps of: depositing the sequence of oligonucleotides on the support surface by printing techniques with or without contact; and anchor oligonucleotide sequence to the support surface by radical addition by reaction TEC TYC caused by photoirradiation with near UV light to visible during a time interval between 5 minutes and 3 hours, without using crosslinkers and / or catalysts .