Method of surface chemical activation of a solid support based on silicon by direct covalent anchoring of at least one nucleic acid biomoleculum. (Machine-translation by Google Translate, not legally binding)

摘要

Surface chemical activation method of a silicon-based solid support by direct covalent attachment of at least one nucleic acid biomolecule. The present invention relates to a method of surface chemical activation by reaction thiol-ene (tec) or thiol-ino (wc) of a solid support on silicon basis by direct covalent attachment of at least one biomolecule which is a sequence of oligonucleotides of dna or rna, where a) the surface of the support is functionalized with alkene or alkyne groups and the biomolecule has a terminal thiol group, and/or b) the surface of the support is functionalized with thiol groups and the biomolecule has an alkene or terminal alkyne group ; said method comprising the steps of: depositing the sequence of oligonucleotides on the surface of the support by means of printing techniques with or without contact; and anchoring the sequence of oligonucleotides to the surface of the support by radical addition by tec or tyc reaction caused by photoirradiation with uv light close to the visible during a time interval comprised between 5 minutes and 3 hours, without the need to use crosslinkers and/or catalysts . (Machine-translation by Google Translate, not legally binding)