Constructs for expressing herbicide tolerance genes, related plants, and related trait combinations

摘要

The subject invention relates in part to constructs for expressing herbicide tolerance genes, related plants, and related trait combinations. Such constructs and plants comprise a gene referred to herein as DSM-2. This gene was identified in Streptomyces coelicolor (A3). The DSM-2 protein is distantly related to PAT and BAR. DSM-2 can be used as a transgenic trait to impart tolerance in plant cells and plants to the herbicidal molecules glufosinate, phosphinothricin, bialaphos, and/or the like. Introduction of this gene into a variety of plants allows for excellent levels of tolerance and/or resistance to the herbicides glufosinate, bialaphos, and other herbicides. Preferred plants include canola and soybeans. The subject invention also relates to combination of the subject herbicide tolerant crop (HTC) traits along with other traits, including other HTC traits (including but not limited to glyphosate tolerance and 2,4-D tolerance), and/or insect resistance (IR) traits in some preferred embodiments. Some preferred stacks of DSM-2 with IR traits are in tobacco and corn. Thus, various uses of DSM-2 genes are included within the scope of the subject invention. Such uses include stacking of a DSM-2 gene with one or more other transgenic traits and introduction of a DSM-2 gene individually into preferred crops.