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USE OF MICROPARTICLE ADDITIVES TO SIMULTANEOUSLY ENABLE ARTIFACT-FREE IMAGE REGISTRATION, AUTO-FOCUSING, AND CHROMATIC ABERRATION CORRECTION IN MICROSCOPY
USE OF MICROPARTICLE ADDITIVES TO SIMULTANEOUSLY ENABLE ARTIFACT-FREE IMAGE REGISTRATION, AUTO-FOCUSING, AND CHROMATIC ABERRATION CORRECTION IN MICROSCOPY
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机译:使用微粒添加剂在显微镜中同时启用无伪像的配准,自动聚焦和色差校正
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摘要
High-contrast, high-density cell-sized microparticles (120) are introduced into a cell (llO)-containing solution prior to the solution being spread onto a planar substrate (100) for imaging. The microparticles facilitate both the process of imager autofocusing and the subsequent registration of multiple images taken of regions of the substrate. The microparticles can further facilitate the correction of chromatic aberration.
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