Process for preparing apolipoprotein A-1 (Apo A-1)

摘要

The present invention relates to processes of obtaining Apo A-1 from an Apo A-1 containing protein fraction (A), comprising suspending- the Apo A-1 containing protein fraction (A) in a buffer solution (B), removing impurities from the suspension while keeping the Apo A- 1 proteins solubilized, followed by precipitating Apo A~ I from the suspension and collecting the Apo A-l precipitate. Apo A-I obtained by such processes, reconstituted HDL obtained from such Apo A-1, and pharmaceutical compositions comprising such Apo A-I and/or reconstituted HDL also are provided.