GENERAL MONOCLONAL ANTIBODY FOR AFRICAN SWINE FEVER VIRUS STRAINS AS WELL AS PREPARATION METHOD THEREFOR AND APPLICATION THEREOF

摘要

Disclosed are a general monoclonal antibody for African swine fever virus strains as well as a preparation method therefor. The method comprises: immunizing a mouse by using a purified p54 immune protein, fusing spleen cells of the immunized mouse with myeloma cells to prepare hybridoma cells, screening with three screening antigens so as to obtain the hybridoma cells that can stably secrete a monoclonal antibody, and then preparing the monoclonal antibody by using an in vivo or in vitro method, wherein the three screening antigens comprise a prokaryotically expressed p54 recombinant protein, an eukaryotically expressed p54 recombinant protein and an artificially synthesized polypeptide shown in Seq ID No.1.