NUCLEUS MARKER WATERSHED TRANSFORMATION-BASED METHOD FOR SPLITTING ADHERED WHITE BLOOD CELLS

摘要

Disclosed is a nucleus marker watershed transformation-based method for splitting adhered white blood cells. First, an original RGB image is inputted, a difficult problem common in processing images of peripheral blood leukocytes and bone marrow leukocytes is discovered; then, HIS and LUV color space and grayscale space transformations are performed on the original image and features of component images of each channel are analyzed; then, threshold segmentation and image subtraction are performed on component B and a grayscale image to acquire a white blood cell image containing some impurities; immediately thereafter, a nucleated white blood cell group is acquired by means of an image enhancement technique to serve as a marked target; and then, morphological operations and watershed transformations are performed on the nucleated white blood cell group and the white blood cell image containing the impurities to remove the impurities to produce a precise white blood cell image and to solve the problem of cell adhesion; and finally, target white blood cells are clipped and transformed to a LUV space, the white blood cell image is clustered from space and color perspectives to produce a precise white blood cell nucleus.