DNA DNA DNA Plastic Microfluidic DNA Chip For Detecting And Scanning DNA of Fish and Shellfish And Detecting Method For DNA Adopting The Same

摘要

More particularly, the present invention relates to a microfluidic DNA chip for detecting and reading DNA of fish and shellfish, and more particularly to a microfluidic DNA chip comprising a lower substrate of a plastic material, an upper substrate and a cover substrate, To a DNA detection method using an electrochemical electron transfer mediator. Accordingly, the present invention provides a method of manufacturing a microfluidic DNA chip for detecting and reading DNA of fisheries products, comprising the steps of: i) fabricating a lower substrate, an upper substrate, and a cover substrate; Ii) immobilizing the probe DNA on the surface of the working electrode in the gold electrode layer of the lower substrate; Iii) bonding the upper substrate to the lower substrate; Iv) fitting a rubber cap into a sample injection port of the cover substrate; And v) placing a bottom surface of a rubber cap protruded from the cover substrate on a sample injection port of the upper substrate, and bonding the upper substrate and the cover substrate to each other, wherein the upper surface of the lower substrate, In the step of fabricating the substrate, the lower substrate is patterned and deposited with a gold electrode layer, the upper substrate has a microfluidic channel formed therein, and a read pin insertion port, a sample injection port, and a sample discharge port are formed, A microfluidic DNA chip and a plastic microfluidic DNA chip using the microfluidic DNA chip for detecting and reading DNA of fish and shellfish. The present invention also provides a DNA detection method using an electrochemical electron transfer mediator for DNA detection and reading of fish and shellfishes, comprising the steps of: (i) injecting a sample into a rubber cap fixed to a sample injection port of a plastic microfluidic DNA chip of the present invention by inserting the sample into a syringe needle step; II) electrochemically detecting a signal on an electrode surface to which probe DNA is immobilized using an electron transfer mediator; III) hybridizing the target DNA; (Iv) detecting a signal electrochemically using an electron transfer mediator on the surface of the electrode on which the DNA is hybridized; V) comparing the electrochemical detection signals, and a DNA detection method using an electrochemical electron transfer mediator for DNA detection and reading of fish and shellfish.