Microfluidic Detection Module For DNA Analysis Using Integrated Microheaters and DNA Microarrays On Plastic Foil

摘要

We report detection of single nucleotide polymorphisms (SNP) by melting curve analysis (MCA) on DNA microarrays in a plastic microfluidic system with integrated heaters using lamination foils. Thin-film copper microheaters were structured on polyethylene napthalate (PEN) foil and DNA microarrays were printed on the heater foil. The system was integrated in a microfluidic channel and successful detection of SNPs was demonstrated. Thermal and electrical characterization of the system was performed proving that the recently implemented low-cost heater encapsulation method using a simple single-sided adhesive foil has not affected the systems performance relative the previous Parylene C encapsulation.